Extraction, Purification and Characterization of a Galactomannan from Prosopis Juliflora (sw.) Dc. Seed Polysaccharides
نویسندگان
چکیده
Prosopis juliflora (Sw.) DC. an Indian fast growing and spreading tree of which pods, leaves are extensively used for various applications. The seeds contain about 22% endosperm and possess the characteristics of becoming a potential source of seed gum. The structural aspects of the galactomannans have been determined for a better understanding of its properties. The purified seed polysaccharide has been characterized as a pure galactomannan having a mannose-galactose ratio of 1:1.4 and the average molecular weight (Mw) is 10.7 x 10 5 D. Partial hydrolysis of the polysaccharide furnished one hepta-(I), one octa-(II) and nona-(III) saccharides. Hydrolysis of oligosaccharide I, II and III followed by GLC analysis furnished D-galactose and D-mannose in the ratio 3:4, 3:5 and 5:4 respectively. Methylation analysis, periodate oxidation, smith degradation and NMR studies confirm that the gum has the basic structure of legume galactomannans with a main chain of (1 4) linked -D-mannopyranosyl units to which galactose units are attached at O-6.
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